اثر قطع عصب سیاتیک و فمورال بر پاسخ زخم دیابتی به روغن ماهی در موش صحرایی

The Effect of Denervation Nerve on Response of In Rat Diabetic Rat to Fish Oil M. Shariati1, M. Khaksari2, D.Shahimoridi1 1- Department of Anatomy, Rafsanjan Medical Faculty, Rafsanjan, Iran 2- Department of Physiology, Rafsanjan Medical Faculty, Rafsanjan, Iran Background: Diabetic foot problems due to angiopathy and neuropathy account for 50% of all nontraumatic amputations and constiute a significant economic burden to society. Our previous study shown that fish oil have therapeutic effect on wound healing in diabetic in rat. The aim of this investigation was to evaluate the effect of denervation on the response of of diabetic rat wounds to fish oil. Material and Methods: This experimental study performed on adult male rats which were divided into 5 groups of ten. Group of normal rats which did not receieved streptozocine (STZ), and the remain four groups became diabetic by the STZ (50 mg/kg, i.p) injection. Diabetic groups divided into two sub groups 2 groups denervated, intact after anesthesia, aseptic denervation were made on the hind limb by sciatic and femoral nerve anatomy. All animals were made wound by a circular 3 cm 2 full thickness incision on hind leg, and receieved topically fish oil on postoperative days. Wounds were made in all animals by a circular 3 cm 2 full thickness incision on hind leg and receieved topically fish oil on postoperative days. Wound surface area and percentage of wound healing were measured at 0, 3, 6, 9, 12, 15 and 18 postoperative days. Time required for healing was also measured. Results: The results showed that the area of the wound in normal group after the days was less than diabetic (P<0.05) and denervated diabetic (P<0.01) groups. Wound area in denervated group was similar to intact group. In the fish oil treated diabetic group, wound surface area, at after 9th day was less than diabetic (P<0.01) and denervated diabetic (P<0.05) groups. Percentage of wound healing in normal group was more than diabetic (P<0.05) and denervated diabetic (P<0.001) groups. Precentage of wound healing increased in the fish oil treated diabetic (P<0.001) and denervated diabetic (P<0.05) groups. Time require for healing in the fish oil treated groups was less than diabetic groups. Conclusion: Our results suggest that topical application of fish oil can accelerate wound healing even in the absense of nerval influences, and this effect was similar to diabetic rats. Key words: Denervation, Neurpathy, Diabetic mellitus, Wound Healing, Fish oil